Rapor Tarihi: 10.04.2026 23:04
| Course Title | Code | Language | Type | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|---|---|
| Immunological Methods | BIO532 | Turkish | Compulsory | 3 + 0 | 3.0 | 8.0 |
| Prerequisite Courses | |
| Course Level | Graduate |
| Mode of delivery | Face to Face |
| Course Coordinator | Dr. Öğr. Üyesi KUBİLAY İNCİ |
| Instructor(s) | Dr. Öğr. Üyesi KUBİLAY İNCİ (Güz) |
| Goals | The aim of this course is to give information about immune system and immunological diagnosis methods. |
| Course Content | 1. To learn general concepts about immunology. 2. To learn cytokines. 3. To learn the antigen and antibody concepts 4. To learn immunological diagnostic methods. |
| # | Öğrenme Kazanımı |
| 1 | Explains the binding kinetics (affinity, avidity) between antigen and antibody |
| 2 | Designs protocols for ELISA (direct, indirect, sandwich, competitive), Western blotting and immunoprecipitation (IP) techniques, and quantitatively analyses the data obtained using these methods. |
| 3 | It optimises the fluorochromes, fixation techniques and signal specificity used in immunofluorescence (IF) and immunohistochemistry (IHC) methods; it interprets protein expression at the tissue level. |
| 4 | It discusses the production processes of polyclonal and monoclonal antibodies, the generation of hybridoma cells, and the standards for the use of these antibodies in diagnostic and therapeutic applications. |
| 5 | It identifies problems such as non-specific binding or low signal levels that arise in experiments and devises solutions by using appropriate control groups (isotype controls, negative/positive controls). |
| Week | Topics/Applications | Method |
|---|---|---|
| 1. Week | Definition of the immune system | Preparation, After Class Study, Research |
| 2. Week | Cells of the immune system | Preparation, After Class Study, Research, Presentation (Preparation) |
| 3. Week | Definition and functions of cytokines | Preparation, After Class Study, Research, Interview |
| 4. Week | Natural immune system | Preparation, After Class Study, Research, Presentation (Preparation), Practice |
| 5. Week | Antigen antibody definition and properties | Presentation (Preparation) |
| 6. Week | Immunological Diagnostic Methods | Preparation, After Class Study, Research, Practice |
| 7. Week | Agglutination and Hemagglutination Tests | Preparation, After Class Study, Research |
| 8. Week | Article time | Preparation, After Class Study, Research, Other Activities, Interview, Presentation (Preparation), Fieldwork, Practice |
| 9. Week | Precipitation Tests | Presentation (Preparation) |
| 10. Week | Turbidimetric and Neutralization Tests | Preparation, After Class Study, Research |
| 11. Week | Western Blot | Other Activities, Presentation (Preparation) |
| 12. Week | Radioimmunological and Enzyme Immunological Tests | Practice |
| 13. Week | Direct and Indirect Immunofluorescence | Interview, Presentation (Preparation), Practice |
| 14. Week | Description of allergy tests and application methods and ELISA test | Research, Other Activities, Practice |
| No | Program Requirements | Level of Contribution | |||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Improve the scientific knowledge in the field of Biology to a more advanced level. Use the advenced knowledge to innovate, interpret the results, to apply the results and to design unique projects. | ✔ | |||||
| 2 | Gain the ability to identify scientific questions, compare, analyze and solve the problems independently. | ✔ | |||||
| 3 | Gain the skills of using the modern laboratory techniques and analysis methods in the field of biology. | ✔ | |||||
| 4 | Gain the ability to get the information by doing research, to evaluate, interpret and apply it. | ✔ | |||||
| 5 | Gain the ability of discussion, synthesis and applying the scientific knowledge. | ✔ | |||||
| 6 | Gain the ability to design experimental studies, do the applications and analyse the complicated results using advanced skills, like critical thinking, problem solving and deciding. | ✔ | |||||
| 7 | Use the scientific knowledge and apply the ability of problem solving, evaluate and analyse advanced concepts in interdiciplinary fields. | ✔ | |||||
| 8 | Gain the scientific knowledge to set up communication with the collegues. Gain the ability to follow and use of the literature. Gain the ability to share or present the results of his/her own studies with the scientists during a scientific conference, workshop or seminar. | ✔ | |||||
| 9 | Participate scientific collaborations effectively and lead a scientific study if necessary. | ✔ | |||||
| 10 | Follow the innovations in the field of biology. Gain the ability to find resources and to use the databases. | ✔ | |||||
| 11 | Find new or strategical approaches to solve an unpredicted or advanced problems. | ✔ | |||||
| 12 | Improve scientific knowledge where there is a little or limited data present. Gain the ability to connect the information from different scientific diciplines. | ✔ | |||||
| 13 | Find unique ideas and get in contact with the professionals in scientific topics using his/her knowledge and skills. | ✔ | |||||
| 14 | Have the ability to read, understand, speak and write a foreign language in order to contact with the scientists from all over the World. | ✔ | |||||
| 15 | Follow novel developments in computational software and hardware systems related with biology, use this knowledge and skill in research. | ✔ | |||||
| 16 | Obey the ethical regulations and watch the ethical issues while designing a scientific study, collecting data, evaluating and publishing them. | ✔ | |||||
| 17 | Adopt life-long learning strategies during performing scientific studies. | ✔ | |||||
| Program Requirements | DK1 | DK2 | DK3 | DK4 | DK5 |
|---|---|---|---|---|---|
| PY1 | 4 | 4 | 4 | 4 | 4 |
| PY2 | 5 | 5 | 5 | 5 | 5 |
| PY3 | 4 | 4 | 4 | 4 | 4 |
| PY4 | 5 | 5 | 5 | 5 | 5 |
| PY5 | 4 | 4 | 4 | 4 | 4 |
| PY6 | 5 | 5 | 5 | 5 | 5 |
| PY7 | 3 | 3 | 3 | 3 | 3 |
| PY8 | 4 | 4 | 4 | 4 | 4 |
| PY9 | 3 | 3 | 3 | 3 | 3 |
| PY10 | 5 | 5 | 5 | 5 | 5 |
| PY11 | 3 | 3 | 3 | 3 | 3 |
| PY12 | 4 | 4 | 4 | 4 | 4 |
| PY13 | 5 | 5 | 5 | 5 | 5 |
| PY14 | 3 | 3 | 3 | 3 | 3 |
| PY15 | 3 | 3 | 3 | 3 | 3 |
| PY16 | 4 | 4 | 4 | 4 | 4 |
| PY17 | 5 | 5 | 5 | 5 | 5 |
| Ders Kitabı veya Notu | Ders Kitabı veya Ders Notu bulunmamaktadır. |
|---|---|
| Diğer Kaynaklar |
|
| ECTS credits and course workload | Quantity | Duration (Hour) | Total Workload (Hour) | |
|---|---|---|---|---|
|
Ders İçi |
Class Hours | 14 | 3 | 42 |
|
Ders Dışı |
Preparation, After Class Study | 14 | 3 | 42 |
| Research | 14 | 3 | 42 | |
| Interview | 14 | 1 | 14 | |
| Presentation (Preparation) | 14 | 1 | 14 | |
|
Sınavlar |
Midterm | 1 | 2 | 2 |
| Homework | 1 | 4 | 4 | |
| Final | 1 | 2 | 2 | |
| Classroom Activities | 14 | 3 | 42 | |
| Total Workload | 204 | |||
| *AKTS = (Total Workload) / 25,5 | ECTS Credit of the Course | 8.0 | ||
