| Course Title | Code | Language | Type | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|---|---|
| Pharmaceutical Toxicology Laboratory | ECZ499 | Turkish | Compulsory | 8. Semester | 0 + 4 | 2.0 | 3.0 |
| Prerequisite Courses | |
| Course Level | Undergraduate |
| Mode of delivery | Lecture, discussion, question and answer, drill and practice |
| Course Coordinator | Dr. Öğr. Üyesi Didem ORAL |
| Instructor(s) | |
| Goals | The aim of the lesson is to teach to the students practical applications of the toxicology discipline |
| Course Content | Introduction to the toxicology laboratory; Laboratory safety and general information. Demonstration: Extraction, Chromatography Demonstration :Experimental animals DUDAM visit Isolation of acidic drugs, which often cause poisoning, from biological samples, determination of basic identification tests and thin-layer chromatography. Isolation of basic drugs, which often cause poisoning, from biological samples, determination of basic identification tests and thin-layer chromatography Quantitative determination of salicylates, which are analgesic drugs that frequently cause poisoning, in biological samples by basic identification tests and qualitative and spectrophotometric methods. Quantitative determination of paracetamol, one of the analgesic drugs that frequently cause poisoning, in biological samples with basic identification tests, qualitative (cresol-ammonia test) and spectrophotometric methods. Qualitative and quantitative determination of warfarin or theophylline, which are drugs with narrow therapeutic window that frequently cause poisoning, in biological sample. Qualitative and quantitative determination of digital glycosides from cardiovascular drugs that frequently cause poisoning in biological sample. Demonstration ; Cytotoxicity assays. Demonstration ; Genotoxicity assays. DUBIT introduction (Examples of analysis with High Pressure Liquid Chromatography, Spectrophotometric Kinetic Measurement). Seminar on toxicological methods used in forensic medicine. |
| # | Öğrenme Kazanımı |
| 1 | Identifying medicinal, poisonous, or narcotic plants |
| 2 | Evaluating the metabolism of the active ingredient in a drug |
| 3 | Identifying and evaluating drug-drug/food/natural medicinal product/disease interactions |
| 4 | Detection and evaluation of side/adverse effects of drugs and vaccines |
| 5 | Evaluating the toxic effects of drugs |
| 6 | Conduct toxicological risk assessments of non-pharmaceutical xenobiotics. |
| 7 | Performing characterization analyses of drug active ingredients/excipients |
| 8 | Using thin-layer chromatography (TLC) in drug analysis |
| 9 | Using the ultraviolet (UV) spectrophotometric method in drug analysis |
| Week | Topics/Applications | Method |
|---|---|---|
| 1. Week | Introduction to the toxicology laboratory; Laboratory safety and general information. | |
| 2. Week | Demonstration: Extraction, Chromatography | |
| 3. Week | Demonstration:Experimental animals | |
| 4. Week | DUDAM visit | |
| 5. Week | Isolation of acidic drugs, which often cause poisoning, from biological samples, determination of basic identification tests and thin-layer chromatography. | |
| 6. Week | Isolation of basic drugs, which often cause poisoning, from biological samples, determination of basic identification tests and thin-layer chromatography | |
| 7. Week | Quantitative determination of salicylates, which are analgesic drugs that frequently cause poisoning, in biological samples by basic identification tests and qualitative and spectrophotometric methods | |
| 8. Week | Midterm exam | Research Preparation, After Class Study Presentation (Preparation) Practice Class Hours |
| 9. Week | Quantitative determination of paracetamol, one of the analgesic drugs that frequently cause poisoning, in biological samples with basic identification tests, qualitative (cresol-ammonia test) and spectrophotometric methods | |
| 10. Week | Qualitative and quantitative determination of warfarin or theophylline, which are drugs with narrow therapeutic window that frequently cause poisoning, in biological sample. | |
| 11. Week | Qualitative and quantitative determination of digital glycosides from cardiovascular drugs that frequently cause poisoning in biological sample. | |
| 12. Week | Demonstration ; Cytotoxicity assays. | |
| 13. Week | Demonstration ; Genotoxicity assays. | |
| 14. Week | DUBIT introduction (Examples of analysis with High Pressure Liquid Chromatography, Spectrophotometric Kinetic Measurement). |
| No | Program Requirements | Level of Contribution | |||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |||
| 1 | Uses the knowledge and skills acquired by completing the pharmacy undergraduate programme in the application areas of the profession by taking initiative when necessary within the framework of ethical and deontological rules in line with the current laws, regulations and legislation. | ||||||
| Program Requirements | DK1 | DK2 | DK3 | DK4 | DK5 | DK6 | DK7 | DK8 | DK9 |
|---|---|---|---|---|---|---|---|---|---|
| PY1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| ECTS credits and course workload | Quantity | Duration (Hour) | Total Workload (Hour) | |
|---|---|---|---|---|
|
Ders İçi |
Class Hours | 14 | 3 | 42 |
|
Sınavlar |
Midterm 1 | 1 | 2 | 2 |
| Final | 1 | 2 | 2 | |
| Practice | 14 | 2 | 28 | |
| Classroom Activities | 3 | 1 | 3 | |
| Total Workload | 77 | |||
| *AKTS = (Total Workload) / 25,5 | ECTS Credit of the Course | 3.0 | ||
