Course Information

Course Information
Course Title Code Semester L+U Hour Credits ECTS
Surgical Methods in Experimental Animals BIO777 3 + 2 4.0 7.5
Prerequisites None
Language of Instruction Turkish
Course Level Graduate
Course Type
Mode of delivery Face to Face
Course Coordinator Assoc. Prof. Dr. Salih Tunç KAYA
Instructors
Assistants
Goals The aim of this course is to provide students with practical general knowledge and skills about basic surgical techniques in experimental animals.
Course Content Laboratory introduction and physical conditions required in the animal laboratory, animal cages, housing, and care, Experimental animal breeds, animal production, animal welfare, Preoperative and postoperative preparation, Use of surgical instruments, Anesthesia and euthanasia techniques, basic suture techniques, marking methods in experimental groups, Anatomy and histology of experimental animals, Experimental Animal Physiology, Ethical concepts in experimental animals and alternative methods to animal studies,Experimental studies planning, Animal diseases, quarantine, internal and external parasites, laboratory safety, Autopsy in experimental animals, Legal regulations in laboratory animals in our country and in the world, Experimental animal biochemistry, Ethics committees and filling out the ethics committee application form
Learning Outcomes - Learn the general characteristics of experimental animals.
- Learn how to work with experimental animals and ethical rules
- Knows drug delivery and injection techniques.
- Practices basic surgical techniques in experimental animals
- Learn how to create and apply experimental models.
Weekly Topics (Content)
Week Topics Learning Methods
1. Week Laboratory introduction and physical conditions required in the animal laboratory, animal cages, housing, and care (T) The functioning of the laboratory for experimental animals, types of animal cages, removing animals from the cage (P) Preparation, After Class Study Practice Verbal Expression Research Course Hours Other Activities Visual Presentation
2. Week Experimental animal breeds, animal production, animal welfare (T) Laboratory animal handling techniques (P) Practice Visual Presentation Preparation, After Class Study Verbal Expression Research Course Hours
3. Week Preoperative and postoperative preparation, Use of surgical instruments (T) Laboratory animal handling techniques (P) Research Preparation, After Class Study Course Hours Verbal Expression Visual Presentation Practice
4. Week Anesthesia and euthanasia techniques, basic suture techniques, marking methods in experimental groups (T) Injection techniques (P) Research Visual Presentation Course Hours Practice Verbal Expression Preparation, After Class Study
5. Week Anatomy and histology of experimental animals (T) Anesthesia and suturing in the rat (P) Course Hours Research Preparation, After Class Study Verbal Expression Visual Presentation Practice
6. Week Experimental Animal Physiology (T) Experimental Models for the Study of Diabetes (P)
7. Week Ethical concepts in experimental animals and alternative methods to animal studies (T) Rat blood collection techniques, heart blood collection technique and tracheostomy (P) Preparation, After Class Study Research Practice Visual Presentation Course Hours Verbal Expression
8. Week Experimental studies planning (T) In vivo tissue perfusion (P) / Mid Term Research Preparation, After Class Study Verbal Expression Visual Presentation Course Hours Practice
9. Week Animal diseases, quarantine, internal and external parasites, laboratory safety (T) Measurement of blood pressure in rat in vivo (P) Course Hours Visual Presentation Research Practice Preparation, After Class Study Verbal Expression
10. Week Autopsy in experimental animals (T). Autopsy (P) Course Hours Preparation, After Class Study Verbal Expression Visual Presentation Practice Research
11. Week Legal regulations in laboratory animals in our country and in the world (T) Hypertension models (P) Course Hours Preparation, After Class Study Visual Presentation Research Practice Verbal Expression
12. Week Experimental animal biochemistry (T) Ovariectomy (P) Course Hours Preparation, After Class Study Verbal Expression Visual Presentation Practice
13. Week Ethics committees and filling out the ethics committee application form (T) Detection of the estrous period in the rat, testicular biopsy and sperm collection, in vivo sperm collection (P) Preparation, After Class Study Research Visual Presentation Practice Verbal Expression Course Hours
14. Week Homework presentations (T) Recording of physiological data in rat: Myocardial ischemia and reperfusion injury, ECG recording (P) Course Hours Preparation, After Class Study Visual Presentation Verbal Expression Practice Research
Recommended Sources
• Principles of Laboratory Animal Science, Revised Edition Edited by L.F.M. van Zutphen, V. Baumans, A.C. Beynen,2001, ELSEVIER, Amsterdam, The Netherlands, ISBN: 0-444-50612-8
• Introduction to Laboratory Animal Science and Technology, J.K. Inglis, Pergamon Press, GB, 1980
• The Design of Animal Experiments, ‎ Royal Society of Medicine Pr Ltd; 1st edition (January 1, 2002), ISBN-10: ‎1853155136
Relations with Education Attainment Program Course Competencies
Program Requirements Contribution Level DK1 DK2 DK3 DK4 DK5 Measurement Method
PY1 5 5 5 5 5 5 -
PY2 5 5 5 5 5 5 -
PY3 5 5 5 5 5 5 -
PY4 5 0 0 0 0 0 -
PY5 5 5 5 5 5 5 -
PY6 5 5 5 5 5 5 -
PY7 5 5 5 5 5 5 -
PY8 4 4 4 4 4 4 -
PY9 5 5 5 5 5 5 -
PY10 5 5 5 5 5 5 -
PY11 5 5 5 5 5 5 -
PY12 3 3 3 3 3 3 -
PY13 5 5 5 5 5 5 -
PY14 2 2 2 2 2 2 -
PY15 2 2 2 2 2 2 -
PY16 5 5 5 5 5 5 -
PY17 4 4 4 4 4 4 -
*DK = Course's Contrubution.
0 1 2 3 4 5
Course's Level of contribution None Very Low Low Fair High Very High
Method of assessment/evaluation Written exam Oral Exams Assignment/Project Laboratory work Presentation/Seminar
ECTS credits and course workload
Event Quantity Duration (Hour) Total Workload (Hour)
Course Hours 14 3 42
Research 14 2 28
Verbal Expression 2 2.625 5.25
Visual Presentation 14 3 42
Practice 14 2 28
Midterm 1 1 2 2
Final 1 2 2
Classroom Activities 14 3 42
Total Workload 191.25
ECTS Credit of the Course 7.5