| Course Title | Code | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|
| Biophysical Research Methods | BIO616 | 3 + 0 | 3.0 | 8.0 |
| Prerequisites | None |
| Language of Instruction | Turkish |
| Course Level | Graduate |
| Course Type | |
| Mode of delivery | Face to face |
| Course Coordinator |
Assoc. Prof. Dr. Gülgün ÇAKMAK ARSLAN |
| Instructors | |
| Assistants | |
| Goals | The aim of this lecture is to learn about determination of acid-base equilibrium parameters, methods for blood gas analysis, measurements of pH, PO2 and PCO2, and the factors affecting the viscosity in blood plasma and serum and microscopic and spectrophotometric methods, electro-physiological and electro-biophysical recording systems, effects of UV and visible light and biomedical applications of laser. |
| Course Content | Determination of acid-base equilibrium parameters; determination of pH, Methods for analysis of blood gases PO2 and PCO2, The factors affecting the viscosity in blood plasma and serum; determination of viscosity, Chromatography and centrifugation techniques, Sedimentation, Microscopic techniques (light, fluorescense, confocale, electron microscopy), Spectroscopic techniques and applications, Electrophoresis, Electrophysiological recording methods, Non-radioactive labelling methods and immunological methods, Measurement of singlet oxygen and photohemolysis, Lasers and biomedical applications of lasers |
| Learning Outcomes |
- To get knowledge about the determination of the acid-base parameters. - To learn the determination of blood gases, pH, PO2 and PCO2 - To learn the microscopic and spectroscopic techniques - To have knowledge about electrophysiological recording methods - To learn non-radioactive labelling methods and immunological methods |
| Week | Topics | Learning Methods |
|---|---|---|
| 1. Week | Determination of acid-base equilibrium parameters; determination of pH | |
| 2. Week | Methods for analysis of blood gases PO2 and PCO2 | |
| 3. Week | The factors affecting the viscosity in blood plasma and serum; determination of viscosity | |
| 4. Week | Chromatography and centrifugation techniques | |
| 5. Week | Sedimentation | |
| 6. Week | Microscopic techniques (light, fluorescense, confocale, electron microscopy) | |
| 7. Week | Spectroscopic techniques and applications | |
| 8. Week | Mid-term exam | |
| 9. Week | Electrophoresis | |
| 10. Week | Electrophysiological recording methods | |
| 11. Week | Non-radioactive labelling methods and immunological methods | |
| 12. Week | Measurement of singlet oxygen and photohemolysis | |
| 13. Week | Lasers and biomedical applications of lasers | |
| 14. Week | Presentation and discussion about scientific papers |
| Ferit Pehlivan, Biyofizik, Hacettepe-Taş yayınevi, Ankara, 2011. |
| Şefik Dursun (ed.) Biyofizik Ders Notları, CTF Yayınevi, İstanbul, 2010. |
| Guyton ve Hall, Tıbbi Fizyoloji, 2010 |
| Program Requirements | Contribution Level | DK1 | DK2 | DK3 | DK4 | DK5 | Measurement Method |
|---|---|---|---|---|---|---|---|
| PY1 | 3 | 0 | 0 | 0 | 0 | 0 | - |
| PY2 | 3 | 0 | 0 | 0 | 0 | 0 | - |
| PY3 | 2 | 0 | 0 | 0 | 0 | 0 | - |
| PY4 | 4 | 0 | 0 | 0 | 0 | 0 | - |
| PY5 | 4 | 0 | 0 | 0 | 0 | 0 | - |
| PY6 | 3 | 0 | 0 | 0 | 0 | 0 | - |
| PY7 | 3 | 0 | 0 | 0 | 0 | 0 | - |
| PY8 | 4 | 0 | 0 | 0 | 0 | 0 | - |
| PY9 | 2 | 0 | 0 | 0 | 0 | 0 | - |
| PY10 | 4 | 0 | 0 | 0 | 0 | 0 | - |
| PY11 | 2 | 0 | 0 | 0 | 0 | 0 | - |
| PY12 | 3 | 0 | 0 | 0 | 0 | 0 | - |
| PY13 | 3 | 0 | 0 | 0 | 0 | 0 | - |
| PY14 | 1 | 0 | 0 | 0 | 0 | 0 | - |
| PY15 | 2 | 0 | 0 | 0 | 0 | 0 | - |
| PY16 | 3 | 0 | 0 | 0 | 0 | 0 | - |
| PY17 | 3 | 0 | 0 | 0 | 0 | 0 | - |
| 0 | 1 | 2 | 3 | 4 | 5 | |
|---|---|---|---|---|---|---|
| Course's Level of contribution | None | Very Low | Low | Fair | High | Very High |
| Method of assessment/evaluation | Written exam | Oral Exams | Assignment/Project | Laboratory work | Presentation/Seminar |
| Event | Quantity | Duration (Hour) | Total Workload (Hour) |
|---|---|---|---|
| Midterm 1 | 1 | 2 | 2 |
| Homework 1 | 1 | 56 | 56 |
| Homework 2 | 1 | 56 | 56 |
| Final | 1 | 2 | 2 |
| Practice End-Of-Term | 1 | 46 | 46 |
| Classroom Activities | 14 | 3 | 42 |
| Total Workload | 204 | ||
| ECTS Credit of the Course | 8.0 | ||
