| Course Title | Code | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|
| Methods Used in Molecular Biology II | BIO542 | 3 + 0 | 3.0 | 8.0 |
| Prerequisites | None |
| Language of Instruction | Turkish |
| Course Level | Graduate |
| Course Type | |
| Mode of delivery | Face to face |
| Course Coordinator |
Prof. Dr. MERAL KEKEÇOĞLU |
| Instructors |
MERAL KEKEÇOĞLU |
| Assistants | |
| Goals | To learn basic techniques used in the field of molecular biology. |
| Course Content | Plasmids and their usefulness in molecular cloning, Bacteriophage λ and its vectors, Bacteriophage M13 vectors, High capacity vectors, Gel electrophoresis, Preparation and Analysis of Eukaryotic Genomic DNA, Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells, Extraction, Purification, and Analysis of proteins from Eukaryotic Cells |
| Learning Outcomes |
- Learn the basic laboratory techniques used in molecular level - Comprehend the application of these methods - Able to design experiments using the basic techniques. |
| Week | Topics | Learning Methods |
|---|---|---|
| 1. Week | The importance and history of markers | |
| 2. Week | Basic concepts and terminology in genotyping | |
| 3. Week | Introduction to genetic markers | |
| 4. Week | RFLP markers | |
| 5. Week | VNTR markers | |
| 6. Week | SSR and ISSR markers | |
| 7. Week | RAPD markers | |
| 8. Week | Midterm | |
| 9. Week | AFLP markers | |
| 10. Week | SSCP and STS markers | |
| 11. Week | SNP markers | |
| 12. Week | Microarray technology | |
| 13. Week | Data analysis in genotyping | |
| 14. Week | Applications of molecular markers |
| Mapping with Molecular Markers, Griffiths AJF, Miller JH, Suzuki DT, W. H. Freeman and Company, USA, 2000. |
| Molecular Marker Systems in Plant Breeding and Crop Improvement, Lorz H, Wenzel G, Springer, USA, 2004. |
| Molecular Cloning: A Laboratory Manual, J. Sambrook, EF. Fritsch, T. Maniatis, Cold Spring Harbor, USA, 1982. |
| Molecular Cloning: A Laboratory Manual, J. Sambrook, EF. Fritsch, T. Maniatis, Cold Spring Harbor, USA, 1982. |
| Program Requirements | Contribution Level | DK1 | DK2 | DK3 | Measurement Method |
|---|---|---|---|---|---|
| PY1 | 4 | 4 | 4 | 4 | 40,60 |
| PY2 | 4 | 4 | 4 | 4 | 40,60 |
| PY3 | 5 | 5 | 5 | 5 | 40,60 |
| PY4 | 4 | 4 | 4 | 4 | 40,60 |
| PY5 | 4 | 4 | 4 | 4 | 40,60 |
| PY6 | 4 | 4 | 4 | 4 | 40,60 |
| PY7 | 4 | 4 | 4 | 4 | 40,60 |
| PY8 | 5 | 5 | 5 | 5 | 40,60 |
| PY9 | 5 | 5 | 5 | 5 | 40,60 |
| PY10 | 4 | 4 | 4 | 4 | 40,60 |
| PY11 | 4 | 4 | 4 | 4 | 40,60 |
| PY12 | 4 | 4 | 4 | 4 | 40,60 |
| PY13 | 5 | 5 | 5 | 5 | 40,60 |
| PY14 | 2 | 2 | 2 | 2 | 40,60 |
| PY15 | 2 | 2 | 2 | 2 | 40,60 |
| PY16 | 4 | 4 | 4 | 4 | 40,60 |
| PY17 | 4 | 4 | 4 | 4 | 40,60 |
| 0 | 1 | 2 | 3 | 4 | 5 | |
|---|---|---|---|---|---|---|
| Course's Level of contribution | None | Very Low | Low | Fair | High | Very High |
| Method of assessment/evaluation | Written exam | Oral Exams | Assignment/Project | Laboratory work | Presentation/Seminar |
| Event | Quantity | Duration (Hour) | Total Workload (Hour) |
|---|---|---|---|
| Course Hours | 14 | 4 | 56 |
| Preparation, After Class Study | 1 | 10 | 10 |
| Research | 1 | 18 | 18 |
| Other Activities | 1 | 8 | 8 |
| Verbal Expression | 14 | 4 | 56 |
| Midterm 1 | 14 | 1 | 14 |
| Homework 1 | 14 | 1 | 14 |
| Final | 1 | 14 | 14 |
| Classroom Activities | 14 | 1 | 14 |
| Total Workload | 204 | ||
| ECTS Credit of the Course | 8.0 | ||
