| Course Title | Code | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|
| Surgical Methods in Experimental Animals | BIO522 | 3 + 2 | 4.0 | 8.0 |
| Prerequisites | None |
| Language of Instruction | Turkish |
| Course Level | Graduate |
| Course Type | |
| Mode of delivery | Face to Face |
| Course Coordinator |
Assoc. Prof. Dr. Salih Tunç KAYA |
| Instructors | |
| Assistants | |
| Goals | The aim of this course is to provide students with practical general knowledge and skills about basic surgical techniques in experimental animals. |
| Course Content | Laboratory introduction and physical conditions required in the animal laboratory, animal cages, housing, and care, Experimental animal breeds, animal production, animal welfare, Preoperative and postoperative preparation, Use of surgical instruments, Anesthesia and euthanasia techniques, basic suture techniques, marking methods in experimental groups, Anatomy and histology of experimental animals, Experimental Animal Physiology, Ethical concepts in experimental animals and alternative methods to animal studies,Experimental studies planning, Animal diseases, quarantine, internal and external parasites, laboratory safety, Autopsy in experimental animals, Legal regulations in laboratory animals in our country and in the world, Experimental animal biochemistry, Ethics committees and filling out the ethics committee application form |
| Learning Outcomes |
- Learn the general characteristics of experimental animals. - Learn how to work with experimental animals and ethical rules - Knows drug delivery and injection techniques. - Practices basic surgical techniques in experimental animals - Learn how to create and apply experimental models. |
| Week | Topics | Learning Methods |
|---|---|---|
| 1. Week | Laboratory introduction and physical conditions required in the animal laboratory, animal cages, housing, and care (T) The functioning of the laboratory for experimental animals, types of animal cages, removing animals from the cage (P) | |
| 2. Week | Experimental animal breeds, animal production, animal welfare (T) Laboratory animal handling techniques (P) | |
| 3. Week | Preoperative and postoperative preparation, Use of surgical instruments (T) Laboratory animal handling techniques (P) | |
| 4. Week | Anesthesia and euthanasia techniques, basic suture techniques, marking methods in experimental groups (T) Injection techniques (P) | |
| 5. Week | Anatomy and histology of experimental animals (T) Anesthesia and suturing in the rat (P) | |
| 6. Week | Experimental Animal Physiology (T) Experimental Models for the Study of Diabetes (P) | |
| 7. Week | Ethical concepts in experimental animals and alternative methods to animal studies (T) Rat blood collection techniques, heart blood collection technique and tracheostomy (P) | |
| 8. Week | Experimental studies planning (T) In vivo tissue perfusion (P) / Mid Term | |
| 9. Week | Animal diseases, quarantine, internal and external parasites, laboratory safety (T) Measurement of blood pressure in rat in vivo (P) | |
| 10. Week | Autopsy in experimental animals (T). Autopsy (P) | |
| 11. Week | Legal regulations in laboratory animals in our country and in the world (T) Hypertension models (P) | |
| 12. Week | Experimental animal biochemistry (T) Ovariectomy (P) | |
| 13. Week | Ethics committees and filling out the ethics committee application form (T) Detection of the estrous period in the rat, testicular biopsy and sperm collection, in vivo sperm collection (P) | |
| 14. Week | Homework presentations (T) Recording of physiological data in rat: Myocardial ischemia and reperfusion injury, ECG recording (P) |
| Experimental Surgical Models in the Laboratory Rat, edited by Alfredo Rigalli and Veronica Elina Di Loreto. CRC Press, London, New York, International Standard Book Number‑13: 978‑1‑4200‑9326‑1, 2009 |
| Principles of Laboratory Animal Science, Revised Edition Edited by L.F.M. van Zutphen, V. Baumans, A.C. Beynen,2001, ELSEVIER, Amsterdam, The Netherlands, ISBN: 0-444-50612-8 |
| Introduction to Laboratory Animal Science and Technology, J.K. Inglis, Pergamon Press, GB, 1980 |
| The Design of Animal Experiments, Royal Society of Medicine Pr Ltd; 1st edition (January 1, 2002), ISBN-10: 1853155136 |
| Program Requirements | Contribution Level | DK1 | DK2 | DK3 | DK4 | DK5 | Measurement Method |
|---|---|---|---|---|---|---|---|
| PY1 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY2 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY3 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY4 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY5 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY6 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY8 | 4 | 4 | 4 | 4 | 4 | 4 | - |
| PY9 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY10 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY11 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY12 | 4 | 4 | 4 | 4 | 4 | 4 | - |
| PY13 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY14 | 2 | 2 | 2 | 2 | 2 | 2 | - |
| PY15 | 2 | 2 | 2 | 2 | 2 | 2 | - |
| PY16 | 5 | 5 | 5 | 5 | 5 | 5 | - |
| PY17 | 4 | 4 | 4 | 4 | 4 | 4 | - |
| 0 | 1 | 2 | 3 | 4 | 5 | |
|---|---|---|---|---|---|---|
| Course's Level of contribution | None | Very Low | Low | Fair | High | Very High |
| Method of assessment/evaluation | Written exam | Oral Exams | Assignment/Project | Laboratory work | Presentation/Seminar |
| Event | Quantity | Duration (Hour) | Total Workload (Hour) |
|---|---|---|---|
| Course Hours | 14 | 3 | 42 |
| Preparation, After Class Study | 14 | 1 | 14 |
| Research | 14 | 3 | 42 |
| Other Activities | 14 | 2 | 28 |
| Verbal Expression | 4 | 1 | 4 |
| Visual Presentation | 14 | 1 | 14 |
| Practice | 14 | 2 | 28 |
| Midterm 1 | 1 | 2 | 2 |
| Final | 1 | 2 | 2 |
| Practice | 14 | 1 | 14 |
| Classroom Activities | 14 | 1 | 14 |
| Total Workload | 204 | ||
| ECTS Credit of the Course | 8.0 | ||
