| Course Title | Code | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|
| Pharmaceutical Toxicology Laboratory 1 | ECZ499 | 7. Semester | 0 + 4 | 2.0 | 2.0 |
| Prerequisites | None |
| Language of Instruction | Turkish |
| Course Level | Undergraduate |
| Course Type | |
| Mode of delivery | Lecture, discussion, question and answer, drill and practice |
| Course Coordinator |
Assist. Prof. Dr. Didem ORAL |
| Instructors | |
| Assistants | |
| Goals | The aim of the lesson is to teach to the students practical applications of the toxicology discipline |
| Course Content | Introduction to the toxicology laboratory; Laboratory safety and general information. Demonstration: Extraction, Chromatography Demonstration :Experimental animals DUDAM visit Isolation of acidic drugs, which often cause poisoning, from biological samples, determination of basic identification tests and thin-layer chromatography. Isolation of basic drugs, which often cause poisoning, from biological samples, determination of basic identification tests and thin-layer chromatography Quantitative determination of salicylates, which are analgesic drugs that frequently cause poisoning, in biological samples by basic identification tests and qualitative and spectrophotometric methods. Quantitative determination of paracetamol, one of the analgesic drugs that frequently cause poisoning, in biological samples with basic identification tests, qualitative (cresol-ammonia test) and spectrophotometric methods. Qualitative and quantitative determination of warfarin or theophylline, which are drugs with narrow therapeutic window that frequently cause poisoning, in biological sample. Qualitative and quantitative determination of digital glycosides from cardiovascular drugs that frequently cause poisoning in biological sample. Demonstration ; Cytotoxicity assays. Demonstration ; Genotoxicity assays. DUBIT introduction (Examples of analysis with High Pressure Liquid Chromatography, Spectrophotometric Kinetic Measurement). Seminar on toxicological methods used in forensic medicine. |
| Learning Outcomes |
- Dersin Öğrenme Çıktıları 1. In general, the basic concepts that should be known for laboratory studies, concentration, density, etc. have knowledge of terms, solution preparation, basic tools used in qualitative and quantitative analysis. 2. To have knowledge about basic tools used in qualitative and quantitative analysis 3. To have knowledge about basic analytical toxicology methods 4. Preparation of biological samples for analysis, analysis and interpretation of results 5. To have knowledge of techniques such as chromatography, extraction, spectroscopy. 6. To have knowledge about toxicology tests such as acute toxicity, cytotoxicity, teratogenicity and genotoxicity. |
| Week | Topics | Learning Methods |
|---|---|---|
| 1. Week | Introduction to the toxicology laboratory; Laboratory safety and general information. | |
| 2. Week | Demonstration: Extraction, Chromatography | |
| 3. Week | Demonstration:Experimental animals | |
| 4. Week | DUDAM visit | |
| 5. Week | Isolation of acidic drugs, which often cause poisoning, from biological samples, determination of basic identification tests and thin-layer chromatography. | |
| 6. Week | Isolation of basic drugs, which often cause poisoning, from biological samples, determination of basic identification tests and thin-layer chromatography | |
| 7. Week | Quantitative determination of salicylates, which are analgesic drugs that frequently cause poisoning, in biological samples by basic identification tests and qualitative and spectrophotometric methods | |
| 8. Week | Midterm exam | |
| 9. Week | Quantitative determination of paracetamol, one of the analgesic drugs that frequently cause poisoning, in biological samples with basic identification tests, qualitative (cresol-ammonia test) and spectrophotometric methods | |
| 10. Week | Qualitative and quantitative determination of warfarin or theophylline, which are drugs with narrow therapeutic window that frequently cause poisoning, in biological sample. | |
| 11. Week | Qualitative and quantitative determination of digital glycosides from cardiovascular drugs that frequently cause poisoning in biological sample. | |
| 12. Week | Demonstration ; Cytotoxicity assays. | |
| 13. Week | Demonstration ; Genotoxicity assays. | |
| 14. Week | DUBIT introduction (Examples of analysis with High Pressure Liquid Chromatography, Spectrophotometric Kinetic Measurement). |
| Program Requirements | Contribution Level | DK1 | Measurement Method |
|---|
| 0 | 1 | 2 | 3 | 4 | 5 | |
|---|---|---|---|---|---|---|
| Course's Level of contribution | None | Very Low | Low | Fair | High | Very High |
| Method of assessment/evaluation | Written exam | Oral Exams | Assignment/Project | Laboratory work | Presentation/Seminar |
| Event | Quantity | Duration (Hour) | Total Workload (Hour) |
|---|---|---|---|
| Midterm 1 | 1 | 1 | 1 |
| Final | 1 | 2 | 2 |
| Practice | 14 | 2 | 28 |
| Practice End-Of-Term | 14 | 1 | 14 |
| Classroom Activities | 3 | 2 | 6 |
| Total Workload | 51 | ||
| ECTS Credit of the Course | 2.0 | ||
