Course Information

Course Information
Course Title Code Language Type Semester L+U Hour Credits ECTS
Animal Tissue Culture BTZ307 Turkish Compulsory 5. Semester 2 + 1 3.0 3.0
Prerequisite Courses
Course Level Undergraduate
Mode of delivery Verbal Expression/Visual Presentation
Course Coordinator Dr. Öğr. Üyesi İlker KILIÇCIOĞLU
Instructor(s)
Goals It aims to inform students about mammalian cell cultures and their use in various applications aimed at working in industrial and research laboratories for various purposes.
Course Content Introduction to cell culture Biology of cells in culture Laboratory design and devices Laboratory safety Containers of culture and psychics Preparation and sterilization Aseptic techniques Primary culture Characterization of cells Viability and cycle analysis Cell freezing Contamination and problem solving Gene transfer to the cell General repetition and discussion
Learning Outcomes
# Öğrenme Kazanımı
1 To learn the analysis methods related to cell viability
2 To learn basic devices and consumables for cell culture
3 To learn the preparation of primary cell lines
4 To learn how to thaw, pass and freeze cells
5 To learn cell culture media
6 To learn the methods of cell characterization analysis and the selection of suitable ones for different purposes
Lesson Plan (Weekly Topics)
Week Topics/Applications Method
1. Week Introduction to cell culture
2. Week Biology of cells in culture
3. Week Laboratory design and devices
4. Week Laboratory safety
5. Week Culture vessels and mediums
6. Week Preparation and sterilization
7. Week Aseptic techniques
8. Week Primary culture
9. Week Characterization of cells
10. Week Viability and cycle analysis
11. Week Cell freezing
12. Week Contamination and problem solving
13. Week Gene transfer to the cell
14. Week General repetition and discussion
*Midterm and final exam dates are not specified in the 14-week course operation plan. Midterm and final exam dates are held on the dates specified in the academic calendar with the decision of the University Senate.
The Matrix for Course & Program Learning Outcomes
No Program Requirements Level of Contribution
1 2 3 4 5
1 .Applies knowledge of natural sciences and mathematics to the development of various processes within the field.
2 Acts in accordance with ethical and deontological principles in decision-making and implementation processes.
3 Utilizes scientific and technological developments in field-related applications.
4 Solves engineering problems within the field through an analytical approach by integrating fundamental engineering knowledge with technical tools.
5 Designs all technical systems, system components, and production processes related to the field.
6 Implements crop and livestock production processes in accordance with scientific and technical principles.
7 Utilizes data-driven core technologies within the agricultural sector in production processes.
8 Applies sustainability principles and approaches to agricultural processes.
9 Utilizes managerial and institutional knowledge for agriculture, taking into account global and local developments.
10 Integrates fundamental scientific knowledge in the fields of genetics, molecular biology, microbiology, and biochemistry into agricultural biotechnology processes through a critical approach.
Relations with Education Attainment Program Course Competencies
Program Requirements DK1 DK2 DK3 DK4 DK5 DK6
PY1 5 5 5 5 5 5
PY2 5 5 5 5 5 5
PY3 4 4 4 4 4 4
PY4 4 4 4 4 4 4
PY5 4 4 4 4 4 4
PY6 5 5 5 5 5 5
PY7 4 4 4 4 4 4
PY8 5 5 5 5 5 5
PY9 3 3 3 3 3 3
PY10 4 4 4 4 4 4
Recommended Sources
Ders Kitabı veya Notu
Diğer Kaynaklar
  • Freshney RI 2005 Culture of Animal Cells: A Manual of Basic Technique. Wiley-Liss: Masters JR. 2000 Animal Cell Culture: A Practical Approach by Oxford University Press, USA Mather JP, Barnes D Animal Cell Culture Methods (Methods in Cell Biology). Academic Press: London.
ECTS credits and course workload
ECTS credits and course workload Quantity Duration (Hour) Total Workload (Hour)
Sınavlar
Midterm 1 1 35 35
Final 1 40 40
Practice 1 1.5 1.5
Total Workload 76.5
*AKTS = (Total Workload) / 25,5 ECTS Credit of the Course 3.0