| Course Title | Code | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|
| Genetic Engineering | ZTB410 | 8. Semester | 2 + 2 | 3.0 | 4.0 |
| Prerequisites | None |
| Language of Instruction | Turkish |
| Course Level | Undergraduate |
| Course Type | |
| Mode of delivery | Face to Face |
| Course Coordinator | |
| Instructors |
Duygu Bircan KADIOĞLU |
| Assistants | |
| Goals | Understanding the basic techniques used by genetic engineering, Contribution of genetic engineering techniques to the analysis of hereditary material, Obtaining information about the use of genetically modified organisms production |
| Course Content | 1-General principles of genetic engineering 2-Learning DNA isolation and cutting enzymes, 3- Understanding recombinant DNA technology and gene transfer methods 4-Polymerase chain reaction (PCR) and DNA-genome sequencing methods |
| Learning Outcomes |
- 1-General principles of genetic engineering - 2-Learning DNA isolation and cutting enzymes, - 3- Understanding recombinant DNA technology and gene transfer methods - 4-Polymerase chain reaction (PCR) and DNA-genome sequencing methods |
| Week | Topics | Learning Methods |
|---|---|---|
| 1. Week | Genetic engineering definition, history and basic principles, gene cloning, what is DNA analysis, introduction to cloning vectors, plasmids, bacteriophages | Visual Presentation Verbal Expression |
| 2. Week | Isolation of genetic material from bacteria, viruses and eukaryotic cells | Visual Presentation Verbal Expression |
| 3. Week | Restriction endonuclease enzyme cleavage, construction of restriction maps, binding of cut fragments (DNAligas enzymes) and properties of other enzymes used in genetic engineering. | Verbal Expression Visual Presentation |
| 4. Week | Cloning vectors (bacteria, yeast, fungi) and gene libraries | Verbal Expression Visual Presentation |
| 5. Week | Cloning vectors (plant, mammalian cell) and gene libraries | Verbal Expression Visual Presentation |
| 6. Week | Natural and artificial transformation techniques (electroporation, protoplast transformation, microinjection, microprojectile bombardment, chemical, viruses etc.) | Verbal Expression Visual Presentation |
| 7. Week | Identification of recombinant cells generated after genetic transfer and development of recombinant clones. | Verbal Expression Visual Presentation |
| 8. Week | MIDTERM EXAM | Course Hours |
| 9. Week | Use of functional complementation tests to identify recombinant clones. | Visual Presentation Verbal Expression |
| 10. Week | Determination of gene locations with chromosome navigation and ectopic binding techniques. | Verbal Expression Visual Presentation |
| 11. Week | Southern, Northern, Western and dot-blot analyzes | Visual Presentation Verbal Expression |
| 12. Week | DNA sequence analyzes (Sanger, next generation sequencing methods) and gene libraries | Verbal Expression Visual Presentation |
| 13. Week | Polymerase chain reaction and its use in molecular diagnosis | Verbal Expression Visual Presentation |
| 14. Week | FINAL EXAM | Course Hours |
| Brown T (Çeviri) Bardakcı F, Yenidünya A.F., Yılmaz N (2009) Gen Klonlama ve DNA Dizi Analizi (GeneCloning and DNA Analysis). ISBN 978-605-395-234-3. |
| Temizkan, G., Arda, N. (2008) Moleküler Biyolojide Kullanılan Yöntemler. Nobel Tıp Kitapevleri. İstanbul Üniversitesi, Biyoteknoloji ve Genetik Mühendisliği Araştırma ve Uygulama Merkezi, (BİYOGEM) Yayın No: 3; ISBN: 978-975-420-583-1 |
| Monika Jain (2012) Recombinant DNA Techniques: A Textbook.Alpha Science. 1st Ed. ISBN-13: 978-1842656679 ISBN-10: 1842656678 |
| Program Requirements | Contribution Level | DK1 | DK2 | DK3 | DK4 | Measurement Method |
|---|---|---|---|---|---|---|
| PY1 | 5 | 5 | 5 | 5 | 5 | 40,60 |
| PY2 | 4 | 4 | 4 | 4 | 4 | 40,60 |
| PY3 | 4 | 4 | 4 | 4 | 4 | 40,60 |
| PY4 | 4 | 4 | 4 | 4 | 4 | 40,60 |
| PY5 | 4 | 4 | 4 | 4 | 4 | 40,60 |
| PY6 | 5 | 5 | 5 | 5 | 5 | 40,60 |
| PY7 | 4 | 4 | 4 | 4 | 4 | 40,60 |
| PY8 | 4 | 4 | 4 | 4 | 4 | 40,60 |
| PY9 | 3 | 3 | 3 | 3 | 3 | 40,60 |
| PY10 | 5 | 5 | 5 | 5 | 5 | 40,60 |
| 0 | 1 | 2 | 3 | 4 | 5 | |
|---|---|---|---|---|---|---|
| Course's Level of contribution | None | Very Low | Low | Fair | High | Very High |
| Method of assessment/evaluation | Written exam | Oral Exams | Assignment/Project | Laboratory work | Presentation/Seminar |
| Event | Quantity | Duration (Hour) | Total Workload (Hour) |
|---|---|---|---|
| Course Hours | 12 | 2 | 24 |
| Preparation, After Class Study | 12 | 1 | 12 |
| Research | 12 | 1 | 12 |
| Practice | 12 | 2 | 24 |
| Midterm 1 | 1 | 3 | 3 |
| Homework 1 | 1 | 12 | 12 |
| Homework 2 | 1 | 12 | 12 |
| Final | 1 | 3 | 3 |
| Total Workload | 102 | ||
| ECTS Credit of the Course | 4.0 | ||
