| Course Title | Code | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|
| Current Methods in Biotechnology | ZTB402 | 8. Semester | 2 + 2 | 3.0 | 5.0 |
| Prerequisites | None |
| Language of Instruction | Turkish |
| Course Level | Undergraduate |
| Course Type | |
| Mode of delivery | Face to Face |
| Course Coordinator | |
| Instructors |
Bahar GEDİK |
| Assistants | |
| Goals | Teaching the current molecular diagnosis and diagnostic methods used in Agricultural Biotechnology together with their applications. |
| Course Content | |
| Learning Outcomes |
- Learning molecular techniques for screening genes and proteins in plants and animals - To learn molecular and serological methods for the detection of pathogens in plants and animals |
| Week | Topics | Learning Methods |
|---|---|---|
| 1. Week | DNA Isolation Methods from Various Plant and Animal Tissues and Polymerase Chain Reaction (PCR). | Visual Presentation Verbal Expression Practice |
| 2. Week | PCR methods and application areas | Verbal Expression Practice Visual Presentation |
| 3. Week | Molecular identification methods after PCR: RFLP, DGGE,TGGE, SSCP | Verbal Expression Visual Presentation Practice |
| 4. Week | DNA Sequencing methods, Sanger DNA Sequencing method, Next Generation DNA Sequencing Method. | Verbal Expression Visual Presentation Practice |
| 5. Week | Real-Time PCR and application areas | Verbal Expression Practice Visual Presentation |
| 6. Week | Microarray Technologies | Practice Visual Presentation Verbal Expression |
| 7. Week | Protein Purification Methods and Quantitation Methods from Various Plant and Animal Tissues | Verbal Expression Visual Presentation Practice |
| 8. Week | MIDTERM EXAM | Course Hours |
| 9. Week | Protein Electrophoresis methods | Practice Visual Presentation Verbal Expression |
| 10. Week | Western Blotting | Visual Presentation Practice Verbal Expression |
| 11. Week | Protein Chips | Visual Presentation Practice Verbal Expression |
| 12. Week | IUD, Elisa and Immunoprecipitation | Verbal Expression Visual Presentation Practice |
| 13. Week | Hybridoma Technologies | Verbal Expression Visual Presentation Practice |
| 14. Week | FINAL EXAM | Course Hours |
| Temizkan G, Arda N 2008, Moleküler Biyolojide Kullanılan Yöntemler, Nobel Tıp Kitapevi, İSTANBUL |
| Guimarães EP, Ruane J, Scherf BD, Sonnino A, Dargie JD 2007 Marker-Assisted Selection. Current Status and Future Perspectives in Crops, Livestock, Forestry and Fish. FAO: Rome, ISBN 978-92-5- 105717-9 |
| Hawley, R.S. And Walker, M.Y. (2003). Advanced Genetic Analysis. Finding Meaning in a Genome. Blackwell Publishing. Victoria/Australia |
| Bartlett JMS, Stirling D (2002).: Methods in molecular Biology-PCR Protocols. Humana Press Newyork /USA |
| Bustin SA 2010.: The PCR Revolution- Basic Technologies and Applications. Cambridge University Press, Cambridge, England. |
| Program Requirements | Contribution Level | DK1 | DK2 | Measurement Method |
|---|---|---|---|---|
| PY1 | 5 | 5 | 5 | 40,60 |
| PY2 | 4 | 4 | 4 | 40,60 |
| PY3 | 5 | 5 | 5 | 40,60 |
| PY4 | 3 | 3 | 3 | 40,60 |
| PY5 | 4 | 4 | 4 | 40,60 |
| PY6 | 3 | 3 | 3 | 40,60 |
| PY7 | 4 | 4 | 4 | 40,60 |
| PY8 | 4 | 4 | 4 | 40,60 |
| PY9 | 1 | 1 | 1 | 40,60 |
| PY10 | 5 | 5 | 5 | 40,60 |
| 0 | 1 | 2 | 3 | 4 | 5 | |
|---|---|---|---|---|---|---|
| Course's Level of contribution | None | Very Low | Low | Fair | High | Very High |
| Method of assessment/evaluation | Written exam | Oral Exams | Assignment/Project | Laboratory work | Presentation/Seminar |
| Event | Quantity | Duration (Hour) | Total Workload (Hour) |
|---|---|---|---|
| Course Hours | 12 | 2 | 24 |
| Research | 12 | 2 | 24 |
| Practice | 12 | 2 | 24 |
| Midterm 1 | 1 | 3.5 | 3.5 |
| Homework 1 | 1 | 24 | 24 |
| Homework 2 | 1 | 24 | 24 |
| Final | 1 | 4 | 4 |
| Total Workload | 127.5 | ||
| ECTS Credit of the Course | 5.0 | ||
