| Course Title | Code | Semester | L+U Hour | Credits | ECTS |
|---|---|---|---|---|---|
| Animal Tissue Culture | ZTB303 | 5. Semester | 2 + 2 | 3.0 | 5.0 |
| Prerequisites | None |
| Language of Instruction | Turkish |
| Course Level | Undergraduate |
| Course Type | |
| Mode of delivery | Verbal Expression/Visual Presentation |
| Course Coordinator |
Assist. Prof. Dr. İlker KILIÇCIOĞLU |
| Instructors |
İlker KILIÇCIOĞLU |
| Assistants | |
| Goals | It aims to inform students about mammalian cell cultures and their use in various applications aimed at working in industrial and research laboratories for various purposes. |
| Course Content | Introduction to cell culture Biology of cells in culture Laboratory design and devices Laboratory safety Containers of culture and psychics Preparation and sterilization Aseptic techniques Primary culture Characterization of cells Viability and cycle analysis Cell freezing Contamination and problem solving Gene transfer to the cell General repetition and discussion |
| Learning Outcomes |
- To learn the analysis methods related to cell viability - To learn basic devices and consumables for cell culture - To learn the preparation of primary cell lines - To learn how to thaw, pass and freeze cells - To learn cell culture media - To learn the methods of cell characterization analysis and the selection of suitable ones for different purposes |
| Week | Topics | Learning Methods |
|---|---|---|
| 1. Week | Introduction to cell culture | |
| 2. Week | Biology of cells in culture | |
| 3. Week | Laboratory design and devices | |
| 4. Week | Laboratory safety | |
| 5. Week | Culture vessels and mediums | |
| 6. Week | Preparation and sterilization | |
| 7. Week | Aseptic techniques | |
| 8. Week | Primary culture | |
| 9. Week | Characterization of cells | |
| 10. Week | Viability and cycle analysis | |
| 11. Week | Cell freezing | |
| 12. Week | Contamination and problem solving | |
| 13. Week | Gene transfer to the cell | |
| 14. Week | General repetition and discussion |
| Freshney RI 2005 Culture of Animal Cells: A Manual of Basic Technique. Wiley-Liss: Masters JR. 2000 Animal Cell Culture: A Practical Approach by Oxford University Press, USA Mather JP, Barnes D Animal Cell Culture Methods (Methods in Cell Biology). Academic Press: London. |
| Document | Ders hocasının notları ve sunumları |
| Program Requirements | Contribution Level | DK1 | DK2 | DK3 | DK4 | DK5 | DK6 | Measurement Method |
|---|---|---|---|---|---|---|---|---|
| PY1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY7 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| PY10 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | - |
| 0 | 1 | 2 | 3 | 4 | 5 | |
|---|---|---|---|---|---|---|
| Course's Level of contribution | None | Very Low | Low | Fair | High | Very High |
| Method of assessment/evaluation | Written exam | Oral Exams | Assignment/Project | Laboratory work | Presentation/Seminar |
| Event | Quantity | Duration (Hour) | Total Workload (Hour) |
|---|---|---|---|
| Midterm 1 | 1 | 35 | 35 |
| Final | 1 | 40 | 40 |
| Practice | 1 | 1.5 | 1.5 |
| Total Workload | 76.5 | ||
| ECTS Credit of the Course | 5.0 | ||
